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pixel intensity quantitation using metamorph 6.1 software  (MetaMorph Inc)

 
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    MetaMorph Inc pixel intensity quantitation using metamorph 6.1 software
    Quantitation of fertilization-induced changes in calcium <t>green</t> <t>fluorescence.</t> Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph <t>6.1</t> software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.
    Pixel Intensity Quantitation Using Metamorph 6.1 Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pixel+intensity+quantitation+using+metamorph+6%2E1+software/pixel+intensity+quantitation+using+metamorph+6+1+software/pmc04077465-113-31-30
    Average 90 stars, based on 1 article reviews
    pixel intensity quantitation using metamorph 6.1 software - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Analysis of Signaling Pathways in Zebrafish Development by Microinjection"

    Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-59745-202-1_6

    Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.
    Figure Legend Snippet: Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.

    Techniques Used: Quantitation Assay, Fluorescence, Injection, Software

    Related Articles

    Fluorescence:

    Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection
    Article Snippet: .. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. ..

    Quantitation Assay:

    Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection
    Article Snippet: .. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. ..

    Software:

    Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection
    Article Snippet: .. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. ..



    Similar Products

    pixel intensity quantitation using metamorph 6.1 software  (MetaMorph Inc)
    90
    MetaMorph Inc pixel intensity quantitation using metamorph 6.1 software
    Quantitation of fertilization-induced changes in calcium <t>green</t> <t>fluorescence.</t> Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph <t>6.1</t> software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.
    Pixel Intensity Quantitation Using Metamorph 6.1 Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pixel+intensity+quantitation+using+metamorph+6%2E1+software/pixel+intensity+quantitation+using+metamorph+6+1+software/pmc04077465-113-31-30
    Average 90 stars, based on 1 article reviews
    pixel intensity quantitation using metamorph 6.1 software - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection

    doi: 10.1007/978-1-59745-202-1_6

    Figure Lengend Snippet: Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.

    Article Snippet: Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software.

    Techniques: Quantitation Assay, Fluorescence, Injection, Software